bTMB-P1 (22.91) Reference Standard

CBP80001-10

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I. Background

Tumor mutational burden (TMB) shows great promise as a biomarker for immuno-oncology (I-O) therapy response, but progress is hindered by the lack of a standardized TMB measurement by targeted NGS panels, and an absence of TMB reference materials.

Sample ID	Cat.No.	Format	Background	Assay	Comments
DC20030079	CBP80001-10N	gDNA	B lymphoblast, Female	500x WES	Same individual
DC20030078	CBP80001-10T	ctDNA (Enzymatic digestion)	stage 4, adenocarcinoma Lung, Female	500x WES	Same individual
IDT xGenExome Research Panel v1.0捕獲，Target Region 39M, Probe Region 51M，CDS Region 33.52M; HiSeq X-TEN，af選擇0.02 cut off，再過濾。 DC20030078 is 100% Tumor sample and DC20030079 is 100% Normal sample.

	Cut off =1%	Cut off =2%	Cut off =3%	Cut off =4%	Cut off =5%
Mutation Number	769	768	734	720	713
TMB value	22.91	22.91	21.90	21.48	21.27
Data Download	DC20030078.filter.0.0.01.tag.maf DC20030078.filter.0.0.02.tag.maf DC20030078.filter.0.0.03.tag.maf DC20030078.filter.0.0.04.tag.maf DC20030078.filter.0.0.05.tag.maf

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