hIL5 Effector Reporter Cell
CBP74182
| I. Background | |
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白細(xì)胞介素 5（IL5）是 Th2（2 型炎癥因子）細(xì)胞因子成員，主要由活化的 Th2、肥 大細(xì)胞及嗜酸性粒細(xì)胞分泌，在嗜酸性粒細(xì)胞的生成、活化及生存調(diào)控中起核心作用。IL5 目前被認(rèn)為是 Th2 通路的關(guān)鍵驅(qū)動因子之一，在自身免疫疾病中扮演重要角色。 |
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| II. Description | |
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IL5 Effector Reporter Cell 報告基因藥靶模型很好的模擬了體內(nèi) IL5 的信號轉(zhuǎn)導(dǎo)過程， 原理見下圖所示。
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| III. Introduction | |
| Expressed gene: |
IL5Rα
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| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+200 μg/ml Hygromycin B+2 μg/ml Puromycin+5 μg/ml Blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
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Figure 2. Recombinant hIL5 Effector Reporter Cell constitutively expressing IL5Rα. |
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Figure 3. Dose Response of Recombinant Human IL5 in IL5 Effector Reporter Cell (C9). |
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Figure 4. Inhibition of hIL5-induced Reporter Activity by Abs in IL5 Effector Reporter Cell (C9). |
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