hNectin4/MC-38
CBPG0035
| I. Background | |
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Nectin 細(xì)胞粘附分子 4 (Nectin4)，是一種 I 型跨膜糖蛋白，屬于 Ig 超家族蛋白中的Nectin 家族。除 Nectin-4 外，還有其他三種 Nectin：Nectin-1、Nectin-2 和 Nectin-3。所有Nectin 都具有相同的結(jié)構(gòu)，由三個細(xì)胞外免疫球蛋白樣結(jié)構(gòu)域（兩個免疫球蛋白樣 (Ig-like)C2 型結(jié)構(gòu)域和一個 Ig-like V 型結(jié)構(gòu)域）、一個跨膜區(qū)和一個胞內(nèi)結(jié)構(gòu)域組成。可溶形式的Nectin-4 蛋白是由金屬蛋白酶 ADAM17/TACE 在細(xì)胞表面進(jìn)行蛋白水解裂解而產(chǎn)生的。 Nectin4 在多種腫瘤細(xì)胞中過表達(dá)，也能促進(jìn)腫瘤細(xì)胞的生長和增殖。高表達(dá) Nectin4的實(shí)體腫瘤包括膀胱癌、胰腺癌、三陰性乳腺癌、非小細(xì)胞肺癌、胃癌、食道癌、卵巢癌等多種癌癥。 |
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| II. Introduction | |
| Host Cell: | MC-38 |
| Expressed gene: | hNectin4 |
| Stability: | 32 passages |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+5μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mouse |
| Tissue: | Colon |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| IV. Representative Data | |
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Figure 1. Recombinant hNectin4/MC38 stably expressing hNectin4. |
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| V. In VIVO Data | |
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Index |
Content |
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Animal |
C57BL/6J, 7weeks, female |
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Implanted Position |
Subcutaneous (right lower flank region ) |
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Injected Cell Numbers |
1×10^6/0.1 ml |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hNectin4/MC38 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3. hNectin4/MC38 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
