hPDL1/B16-F10
CBPG0060
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產(chǎn)品描述
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| I. Background | |||
| Programmed Cell Death Protein 1（PD-1） ，一種在激活的 T 細(xì)胞上表達(dá)的受體，與其 配體 PD-L1 和 PD-L2 結(jié)合，負(fù)向調(diào)節(jié)免疫反應(yīng)。 PD-1 配體存在于大多數(shù)癌癥中，PD- 1:PD-L1/2 相互作用會抑制 T 細(xì)胞活性，并使癌細(xì)胞逃避免疫監(jiān)視。 PD1/PDL1 信號轉(zhuǎn)導(dǎo)通 路是腫瘤免疫抑制的重要組成部分，可以抑制 T 淋巴細(xì)胞的興奮，增強腫瘤細(xì)胞的免疫耐 受，從而實現(xiàn)腫瘤免疫逃逸。PD1 與 PDL1 結(jié)合可以減弱 T 細(xì)胞介導(dǎo)的免疫監(jiān)視，導(dǎo)致免 疫反應(yīng)缺失，甚至導(dǎo)致 T 細(xì)胞凋亡。PD1/PDL1 抑制劑可解除抗腫瘤 T 細(xì)胞的免疫抑制， 從而導(dǎo)致 T 細(xì)胞增殖并滲透到腫瘤微環(huán)境中并誘導(dǎo)抗腫瘤反應(yīng)。PD-1:PD-L1/2 通路還參與 調(diào)節(jié)自身免疫反應(yīng)，使這些蛋白質(zhì)成為多種癌癥以及多發(fā)性硬化癥、關(guān)節(jié)炎、狼瘡和 I 型 糖尿病的有希望的治療靶點。 | |||
| II. Introduction | |||
| Host Cell: | B16-F10 | ||
| Expressed gene: | hPDL1 | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | DMEM+10%FBS+5 μg/ml Blasticidin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mus musculus, mouse | ||
| Tissue: | skin | ||
| Morphology: | mixture of spindle-shaped and epithelial-like cells | ||
| Growth Properties: | adherent | ||
| IV. Representative Data | |||
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Figure 1. Recombinant hPDL1/B16-F10 stably expressing hPDL1. |
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| V. In VIVO Data | |||
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Index |
Content |
|
Animal |
C57BL/6J, 8weeks, female |
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Implanted Position |
Subcutaneous (right lower flank region ) |
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Injected Cell Numbers |
2×10^5/0.1ml |
|
Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hPDL1/B16-F10 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3.hPDL1/B16-F10 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
