ICOS Effector Reporter Cell
CBP74491
詢 價(jià)
索取Protocol
產(chǎn)品描述
產(chǎn)品數(shù)據(jù)庫
| I. Background | |
| ICOS 是一種在 T 細(xì)胞活化后才被誘導(dǎo)表達(dá)的共刺激受體，而 ICOSL 是其配體，主要 組成性表達(dá)于 B 細(xì)胞和抗原呈遞細(xì)胞表面。兩者的核心作用機(jī)理在于，當(dāng)活化的 T 細(xì)胞 （特別是濾泡輔助性 T 細(xì)胞）遷移至淋巴濾泡并與 B 細(xì)胞相遇時(shí)，ICOS 與 ICOSL 的結(jié)合 為 T 細(xì)胞提供了關(guān)鍵的共刺激信號，這主要通過激活 PI3K 等下游通路，驅(qū)動 T 細(xì)胞增殖、 存活及產(chǎn)生細(xì)胞因子（如 IL-21），從而精確調(diào)控生發(fā)中心反應(yīng)，最終促進(jìn) B 細(xì)胞進(jìn)行抗 體親和力成熟與類別轉(zhuǎn)換，產(chǎn)生高效體液免疫。 | |
| II. Description | |
ICOS Effector Reporter Cell 報(bào)告基因藥靶模型很好的模擬了體內(nèi) ICOS 的信號轉(zhuǎn)導(dǎo)過程， 原理見下圖所示。 Figure 1. ICOS Effector Reporter Cell 細(xì)胞模型原理圖 |
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| III. Introduction | |
| Expressed gene: | ICOS |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | RPMI-1640 + 10%FBS + 800 μg/ml Hygromycin B+10 μg/ml Blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
 Figure 2. Recombinant ICOS Effector Reporter Cell stably expressing ICOS.  Figure 3. Dose Response of ICOS Agonist Abs in ICOS Effector Reporter Cell(C3) with FCGR2B TCR Activitor CHO(C18).
 Figure 4. Blocking of ICOSL induced ICOS Effector Reporter Cell(C3) Activity by ICOSL Blocking Ab with ICOSL aAPC CHO(C42). |
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