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LC-2/ad(人肺癌腺癌細胞)

CBP60104

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I. General information 
Synonyms: LC-2/ad
Background: Established from the pleural effusion of a pulmonary adenocarcinoma in a 51 year-old Japanese female. LC-2/ad cells exhibit an epithelial appearance and a tendency to form small domes. Immunocytochemically the cells are positive for CEA and cytokeratins, including cytokeratin No.18. LC-2/ad produces at least two functionally active trypsin inhibitors together with several proteinases in vitro.
Species: human (Homo sapiens)
Tissue: Lung
Disease: Human lung adenocarcinoma
Gender: 51 year-old Japanese female
Morphology: Epithelial
Growth Mode: Adherent
Doubling Time: N/A
DNA Profile: Amelogenin: X, X
CSF1PO: 10,11
D13S317: 10,12
D16S539: 9
D5S818: 10
D7S820: 11
THO1: 7
TPOX: 11
vWA: 16,19
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Culture Medium:

RPMI-1640: F12 (1:1) + 2mM Glutamine+10%FBS

LC-2/ad完全培養(yǎng)基,# CBP60104M
We strongly suggest to purchase the complete medium from us.

Cryopreservation medium: 90%FBS+10%DMSO
Karyotype: Modal No 53-56
Subculture Routine: This cell line is relatively slow growing taking about 5 days to reach 70 -80% confluency. Split cultures 1:2-1:4 ie seeding at 2-5 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/ EDTA ; 5%CO2; 37°C
Comments: For more information, please contact us (4008-750-250).

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